|
KMID : 0364820230590030173
|
|
Korean Journal of Microbiology
2023 Volume.59 No. 3 p.173 ~ p.183
|
|
|
Genetic relationship between inner membrane protein LapB and penicillin-binding protein 1b or YhcB in Escherichia coli
|
|
Lee Han-Byeol
Park Si-Hyoung
Lee Chang-Ro
|
|
|
Abstract
|
|
|
|
The inner membrane protein LapB participates in the regulation of proteolysis of lipopolysaccharide (LPS) biosynthesis protein LpxC by the essential membrane protease FtsH. However, the presence of the LpxC-independent phenotype in the lapB mutant implies an additional physiological role of LapB. In this study, we present novel genetic evidence for other cellular functions of LapB in Escherichia coli. We identified several novel phenotypes of the lapB mutant, such as growth defects under energy-poor carbon sources, and demonstrated that most phenotypes were suppressed by the depletion of penicillin-binding protein 1b (PBP1b), an inner membrane protein that participates in peptidoglycan biosynthesis, in an LpxC-independent manner. Additionally, most phenotypes of the lapB mutant were also suppressed by the depletion of YhcB, an inner membrane protein that is associated with the coordinated biosynthesis of peptidoglycan and LPS. Collectively, our genetic experiments imply that LapB may be associated with the regulation of peptidoglycan biosynthesis, as well as LpxC-mediated modulation of LPS levels.
|
|
|
KEYWORD
|
|
|
inner membrane protein, LapB, lipopolysaccharide, penicillin-binding protein, peptidoglycan, suppression, YhcB
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|